FACILITIES & INSTRUMENTATION

AB SCIEX 4000 QTrap UPLC/MS/MS coupled with Shimadzu 30A UPLC Nexera LC System

Key Features

  • Robust hybrid triple quadruple/linear ion trap mass spectrometer
  • High-pressure tolerance
  • Fast, better separation

Application

  • Study physical, chemical, or biological properties of a variety of compounds
  • Drug discovery and development
  • Metabolite identification and quantitation
  • Proteomic applications (post-translationally modified protein discovery/protein identification)
  • Biomarker validation (mass range, 5-2800 m/z.)

 

AB SCIEX QTrap 6500 UPLC-MS/MS coupled with Shimadzu Nexera X2 UHPLC System

Key Features

  • Scan rates of up to 20,000 Da/sec
  • 20ms polarity switch
  • Quantitative MRM3 workflows
  • Linear ion trap capabilities
  • Detection of analytes with rapid Data Dependent Acquisition (DDA)
  • Data Independent Acquisition (DIA) strategy, SWATH technology

Application

  • Include all capabilities of AB SCIEX 4000 qtrap UPLC/MS/MS system
  • Achieve lowest limits of quantitation in complex matrices
  • Obtain comprehensive peptide sequence confirmation and simplify MRM assay development of peptide quantification
  • Simultaneously quantitate mrms and perform full library scans to search for contaminants

 

SCIEX X500B QTOF System 

Key Features

  • Robust, high-performance high-resolution MS/MS system
  • High mass resolution (>30,000 resolution)
  • High mass accuracy (<1ppm accuracy for m/z>50amu)
  • Removal of interference from complex sample matrices
  • Quantify linearly up to 4 orders of magnitude
  • Identify analytes based on MS/MS (IDA and SWATH™ MS/MSALL, ion ratios and MS/MS spectra)

Application

  • Identify metabolites
  • Analyze larger molecule

 

Waters HPLC and UPLC systems

Key Features

  • Three different Waters models coupled with a broad range of detectors – PDA, dual λ absorbance, UV/VIS, and fluorescence
  • Enhanced separation resolution
  • Increased sample through-put

Application

  • Drug discovery
  • Analyze drugs/metabolites in biological samples.
  • In-vitro diagnostic test to analyze compounds (diagnostic indicators/ therapeutically monitored compounds)